Journal of Analytical and Pharmaceutical Chemistry
ISSN: 2381-8913Abstract
Purpose: Recent research work elaborated palynological study of some selected species of family Lamiaceae which was conducted in District Bhimber, Azad Kashmir. Methods: The selected 6 species of family Lamiaceae were collected from District Bhimber. The flowers were plucked from the plants. The anthers were spaced apart and kept in a glass tube having acetic acid. The anthers were poisoned with the glass rod to set a homogeneous mixture and then purified it. Then slides were prepared and purified and add 1% safranin to stain the slides and study these slides under light microscope, and then add some drops of mixture on slides, which were covered with cover slip.
Introduction
The Lamiaceae family is by far the most prevalent family in practically all global habitats. The Lamiaceae family is the biggest of the Angiosperm families. The family has roughly 236 genera and 7,000 species spread over the globe. Family is incredibly important economically [1]. Asia's primary hub for salvia variety is Anatolia. 95 Salvia species can be found in Turkey, and 49 (52%) of them are indigenous [2]. The vast Lamiaceae (formerly Labiatae) family is widespread, with particularly rich variety in California, Mexico, and the Mediterranean area. Although many are drought-adapted, the plants may be found in a wide range of settings. As a triplet of characters with vegetative traits, this family is one of the few that can typically be identified. The fresh growth (not the old growth) of mints has square stems, and like many other families, mints have opposing leaves. The aromatic leaves of mints, which are also present in numerous other families, are what distinguish them from other plants [3].
Materials and Methods
The pollen slides were prepared from flowers of some plant species collected during field trips. The anthers were placed apart and kept in a tube having a solution of acetic acid and sodium chlorate (NaCIO3 ) in ratios of 1:2. The anthers were poisoned with the glass rod and then purified it. Slides were prepared and purified with glycerol jelly mixed with 1% safranin to stain the slides and study these slides under a light microscope, then a some drops of the mixture were placed on the slides, which were put down on a small section of simple fuchsinstained jelly. Then must b
Results and Discussion
The quantitative characters of pollens were documented with morphometric observation mentioned in Table 2. It was indicated that highest colpus length was measured against Ocimum basilicum (38.73µm) while minimum colpus length was measured against plant Leucas aspera (13.33µm). Mentha longifolia and Ocimum basilicum indicated maximum number (6) of Colpi as compared to other four observed plants (3). The polar axis measurement was also highest (56.63µm) Ocimum basilicum and Leucas aspera expressed lowest (17.15µm) polar axis value. The equatorial axis value was maximum (55.81µm) shown by Ocimum basilicum plant while minimum equatorial axis value (11.66µm) was measured against Leucas cephalotes.
Conclusion
It was concluded that the qualitative study explored the shape of pollen grains which were prolate, spheroidal and sub-spheroidal as it expressed no prominent change in pollen shapes of six studied taxa. The greatest size of pollen 38.73 was reported in Ocimum basilicum and the shortest 20.41 in Men- Submit your Manuscript | www.winnerspublishing.com Winners J Allergy 9(1): id1044 (2023) - Page - 05 Winners Publishing Group tha longifolia. It was finally concluded that a lot of variations in characters were observed in all characters of six plant species. It means that the paleontological study depicted lot of diversity among plants species as indicated Ocimum basilicum to Leucas aspera among observed characters. This study will help us to explore distinctive taxonomic characters of family Lamiaceae.
References
1. Michel J, Abd Rani NZ, Husain K. A review on the potential use of medicinal plants from Asteraceae and Lamiaceae plant family in cardiovascular diseases. Front Pharmacol. 2020; 11: 852. 2. Kahraman A, Dogan M, Celep F, Akaydin G, Koyuncu M. Morphology, anatomy, palynology and nutlet micromorphology of the rediscovered Turkish endemic Salvia ballsiana (Lamiaceae) and their taxonomic implications. Nord J Bot. 2010; 28: 91-9. 3. Silva H. A descriptive overview of the medical uses given to Mentha aromatic herbs throughout history. Biology. 2020; 9: 484. 4. Mahmood A, Mahmood A, Shaheen H, Qureshi RA, Sangi Y, Gilani SA. Ethno medicinal survey of plants from district Bhimber Azad Jammu and Kashmir, Pakistan. J Med Plants Res. 2011; 5: 2348-60. 5. Arslan I, Celik A. Chemical composition and antistaphylococcal activity of an endemic Salvia chrysophyllaStapf naturally distributed Denizli Province (Turkey) and its vicinity. Pak J Bot. 2008; 40: 1799-804